:: proteabio.com - Resources :: Tools - Table of Mass Spec Grade Proteases

Proteomics Tools

Table of Mass Spec Grade Proteases

Enzyme	Class	Cleavage Sites and Digestion Conditions
Trypsin	Serine Protease	Carboxyl side of arginine (R) and lysine (K) Ammonium bicarbonate buffers (pH 8.0). 1:20 to 1:100 (w/w) enzyme to protein ratio Digestion at 37°C for 2 hours to overnight
Endoproteinase Glu-C	Serine Endoproteinase	Carboxyl side of glutamate (G). Ammonium bicarbonate buffers (pH 7.8) Carboxyl side of glutamine (G) and asparagine (N) Phosphate buffers (pH 4.0) 1:20 to 1:100 (w/w) enzyme to protein ratio Digestion at 37°C for 2 hours to overnight
Pepsin	Digestive Protease	Carboxyl side of tyrosine (Y), tryptophan (W), phenylalanine (F), and leucine (L) Acidic conditions (pH 2-4) 1:50 (w/w) enzyme to protein ratio Digestion at 37°C for 6 hours to overnight
Chymotrypsin	Digestive Protease	Carboxyl side of tyrosine (Y), phenylalanine (F), and tryptophan (W) Ammonium bicarbonate buffers (pH 8) 1:20 to 1:100 (w/w) enzyme to protein ratio Digestion at 37°C for 2 hours to overnight
Endoproteinase Arg-C	Sulfhydryl Proteinase	Carboxyl side of arginine (R) residues Ammonium bicarbionate buffers (pH 8) 1:10 to 1:20 (w/w) enzyme to protein ratio Digestion at 37°C overnight

…innovations for bioanalytics

SERVICES