Proteomics Tools
Table of Mass Spec Grade Proteases
Enzyme | Class | Cleavage Sites and Digestion Conditions |
| Trypsin | Serine Protease | Carboxyl side of arginine (R) and lysine (K) Ammonium bicarbonate buffers (pH 8.0). 1:20 to 1:100 (w/w) enzyme to protein ratio Digestion at 37°C for 2 hours to overnight |
| Endoproteinase Glu-C | Serine Endoproteinase | Carboxyl side of glutamate (G). Ammonium bicarbonate buffers (pH 7.8) Carboxyl side of glutamine (G) and asparagine (N) Phosphate buffers (pH 4.0) 1:20 to 1:100 (w/w) enzyme to protein ratio Digestion at 37°C for 2 hours to overnight |
| Pepsin | Digestive Protease | Carboxyl side of tyrosine (Y), tryptophan (W), phenylalanine (F), and leucine (L) Acidic conditions (pH 2-4) 1:50 (w/w) enzyme to protein ratio Digestion at 37°C for 6 hours to overnight |
| Chymotrypsin | Digestive Protease | Carboxyl side of tyrosine (Y), phenylalanine (F), and tryptophan (W) Ammonium bicarbonate buffers (pH 8) 1:20 to 1:100 (w/w) enzyme to protein ratio Digestion at 37°C for 2 hours to overnight |
| Endoproteinase Arg-C | Sulfhydryl Proteinase | Carboxyl side of arginine (R) residues Ammonium bicarbionate buffers (pH 8) 1:10 to 1:20 (w/w) enzyme to protein ratio Digestion at 37°C overnight |
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