It is important to pay careful attention not to damage the cells before the actual cell lysis step.  Severe sample loss can occur by damaging the cells prior to lysis.  During th…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

The presence of urea, ampholytes and glycerol is problematic for TiO₂ selective enrichment. There has to be some form of sample clean-up before en…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

For our Titanium Dioxide ProteaTips (SP-124 and SP-125), it is best to go up and down very slow while pipetting.  The slower the better, because the phosphopeptides have a weak interaction …
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

All of our SpinTip products (SP-150, SP-151, SP-152, SP-155, and SP-154) have the fritted tips.  The SpinTip protocols recommend centrifugation at 4,000 rpm for 3 minutes. The frits are tes…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

When using Glu-C, acetonitrile cannot be used during digestion. The presence of the organic solvent will denature the enzyme and yield poor digestion.  Our digestion buffer contains 1% acet…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

Q: Which Polymeric Monolith Chemistry is appropriate for my sample? Should I use the single use tips, the chromatographic columns, or the plates for my sample(s)?

A: Single tips are u…
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author: haddon.goodman | date created: 03/01/2011 | comments: 0

It may be necessary to desalt samples using a centrifugal ultrafiltration unit (SP-021 and SP-022) to remove serum salts, buffer salts and small molecular weight species from the sample.

We recommend no more than 10 μL of serum (diluted to 400 μL with our activation buffer) be added to the SpinTube.

This is due to the fact that your proteins are no longer being solubilized by the surfactant present. Instead of degrading the surfactant you may need to try an alternative clean-up method, poss…
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author: haddon.goodman | date created: 02/14/2011 | comments: 0

The surfactants are organic molecules, so they can be combusted if the heat is too high (somewhere higher than 200 °C – we have never tested it). Heating the surfactants in an aqueous …
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author: haddon.goodman | date created: 02/14/2011 | comments: 0

There is a white precipitate forming which is the surfactant triethylammonium salt. The triethylammonium cation adds six hydrophobic carbons to the surfactant anion which already has twelve hydr…
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author: haddon.goodman | date created: 02/14/2011 | comments: 0