Protein Gel Stains
Protea’s stains are an excellent choice for visualizing proteins separated by SDS-PAGE gel electrophoresis. Try our Coomassie Blue stains for general visualization, or for more sensitive visualization, try our Zinc or Silver stains. All stains are excellent choices for you electrophoresis needs.
Differentiation between Protea SuperBlue Coomassie Stains
Protea’s SuperBlue™ Coomassie stains are an excellent choice for visualizing proteins separated by SDS-PAGE gel electrophoresis. SuperBlue Coomassie Stains, R250 and G250 stains are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis. The SuperBlue Coomassie Stain, R250 (red-tinted) form lacks two methyl groups that are present in the SuperBlue Coomassie Stain, G250 (green-tinted) form, which is also called colloidal Coomassie dye.
MS Compatibility of Protea Silver Stain Kit and ProteaGel Solutions
Silver staining of proteins is a useful technique for the detection of minor protein components that are difficult to visualize by standard Coomassie blue stains. Silver stains are 50 to 100-fold more sensitive than conventional Coomassie stains and thus capable of detecting low nanogram quantities of protein on gels. The Protea Silver Stain formulation is unique in that the staining process can be completed in about 35 minutes (including the 15 min. fixing step) without sacrificing the sensitivity of detection. In addition, the silver stain kit is compatible with MALDI analysis.