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Protein Gel Separation and Recovery (click a post below to read)

What is the difference between your Coomassie G250 and R250 products and how much protein can they detect in the gel?

Super Blue Coomassie R250 and G250 stains are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electro…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

Why are bands not visible after staining with Coomassie/Silver/Zinc stain?

Not enough protein is present in the gel to be detected.

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author: haddon.goodman | date created: 03/17/2011 | comments: 0

When using the zinc stain, why does my entire gel turn white?

The gel was left in Reagent B for too long; Not enough stop (H₂O) was added to stop the reaction from occurring; No protein is in the gel; The gel…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

When pouring polyacrylamide protein gels, why do I get webbing in the wells and why does it take a long time for the wells to polymerize?

The amount of TEMED added may need to be altered. If webbing is observed, lower the amount of TEMED. If it takes a long time to polymerize, increase the amount of TEMED. The ty…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

How critical are the incubation times when using the Silver Stain Kit?

In order to maintain gel-to-gel reproducibility, incubation times and rinse times should be carefully controlled.

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author: haddon.goodman | date created: 03/17/2011 | comments: 0

Why does my silver stain take an extremely long time to develop

There is probably too much water being used in between reagents A, B, and C. Less than 10 mL of water should be used and wash times should be less than 2-3 seconds. There is also the…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

Do you have precast gels?

Yes. We offer precast mini gels that fit the Bio-Rad and Invitrogen mini gel systems. The gels are currently offered in 7.5%, 10%, 12%, and 15%. Comb options included are 10 we…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

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