:: proteabio.com – Resources :: Tech Tips – Protein Gel Separation and Recovery

Super Blue Coomassie R250 and G250 stains are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electro…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

Not enough protein is present in the gel to be detected. 

The gel was left in Reagent B for too long; Not enough stop (H₂O) was added to stop the reaction from occurring; No protein is in the gel; The gel…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

The amount of TEMED added may need to be altered.  If webbing is observed, lower the amount of TEMED.  If it takes a long time to polymerize, increase the amount of TEMED.  The ty…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

In order to maintain gel-to-gel reproducibility, incubation times and rinse times should be carefully controlled.

There is probably too much water being used in between reagents A, B, and C.  Less than 10 mL of water should be used and wash times should be less than 2-3 seconds.  There is also the…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0

Yes.  We offer precast mini gels that fit the Bio-Rad and Invitrogen mini gel systems.  The gels are currently offered in 7.5%, 10%, 12%, and 15%.  Comb options included are 10 we…
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author: haddon.goodman | date created: 03/17/2011 | comments: 0