Coated Capillaries for CE
Instructions for Use

Protea's Coated Capillaries allow sample separation without interference from capillary EOF.  Our stable coatings also minimize adsorption of sample to the capillary walls, resulting in minimum sample loss and reproducible CE separations.

1.)  Handle the capillary very carefully after removal from the storage pack.

2.)  Cut the capillary to the desired length using a capillary cutter. Measure the distance to the point where window is desired. Create a window by removing the polyimide coating on the outside, using either of the two methods: 1) using a drop of fuming sulfuric acid or 2) Using a low temperature flame. Remove the burned portion using a Kim-Wipe dipped in methanol or acetone. An ideal window is not more than 3 mm long. Once the window is generated in the capillary, be very careful while handling it. It is easy to break the capillary at the window because the glass is now exposed.

3.)  Arrange the capillary in your capillary electrophoresis set-up.

4.)  To condition the capillary, wash it thoroughly with deionized water, using a 5-10 minute rinse. At an injection pressure of 20 psi, 5 minutes rinse is sufficient. Now rinse the capillary thoroughly with the experimental buffer, at least for 15 minutes.

5.)  The capillary is ready for separation experiments. Make sure to rinse the capillary in between runs with the separation buffer or run buffer.

6.)  To store the capillary, rinse the capillary with the run buffer for at least 15 minutes. Then rinse the capillary for 5-10 minutes with deionized water. Blow air through the capillary to dry it thoroughly and store it away. If storing for a long time, blow argon (or nitrogen) through the capillary and store it in an inert atmosphere.

7.)  DO NOT RINSE THE CAPILLARY WITH SODIUM HYDROXIDE OR OTHER STRONG BASES AT ANY POINT DURING ITS USE. RINSING WITH SODIUM HYDROXIDE COMPROMISES THE PERFORMANCE OF THE CAPILLARY.

8.)  The capillary works best at pH between 3.0 and 9.0.