Coated Capillaries for CE
Instructions for Use
Protea's Coated
Capillaries allow sample separation without interference from
capillary EOF. Our stable coatings also minimize adsorption of
sample to the capillary walls, resulting in minimum sample loss and
reproducible CE separations.
1.) Handle the capillary very carefully after removal from the
storage pack.2.) Cut the capillary to the desired length
using a capillary cutter. Measure the distance to the point where
window is desired. Create a window by removing the polyimide coating
on the outside, using either of the two methods: 1) using a drop of
fuming sulfuric acid or 2) Using a low temperature flame. Remove the
burned portion using a Kim-Wipe dipped in methanol or acetone. An
ideal window is not more than 3 mm long. Once the window is generated
in the capillary, be very careful while handling it. It is easy to
break the capillary at the window because the glass is now exposed.
3.) Arrange the capillary in your capillary
electrophoresis set-up.
4.) To condition the capillary, wash it thoroughly with
deionized water, using a 5-10 minute rinse. At an injection
pressure of 20 psi, 5 minutes rinse is sufficient. Now rinse the
capillary thoroughly with the experimental buffer, at least for 15
minutes.
5.) The capillary is ready for separation experiments. Make
sure to rinse the capillary in between runs with the separation
buffer or run buffer.
6.) To store the capillary, rinse the capillary with the run
buffer for at least 15 minutes. Then rinse the capillary for 5-10
minutes with deionized water. Blow air through the capillary to
dry it thoroughly and store it away. If storing for a long time,
blow argon (or nitrogen) through the capillary and store it in an
inert atmosphere.
7.) DO NOT RINSE THE CAPILLARY WITH SODIUM HYDROXIDE OR
OTHER STRONG BASES AT ANY POINT DURING ITS USE. RINSING WITH
SODIUM HYDROXIDE COMPROMISES THE PERFORMANCE OF THE CAPILLARY.
8.) The capillary works best at pH between 3.0 and 9.0.
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