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SEPARATE

The Explorer system is designed to run at both micro and nano flow rates. Both protein and peptide separation columns are available. Other types of chromatographic separations, such as 2D MudPIT, are also supported.
These columns offer the researcher unparalled access to choosing the type of separation and switching between applications. Coupled with the DIGESTor’s in-line digestion capability, the Explorer system offers true simultaneous top-down and bottom-up potential for ground breaking research into biomarker discovery.

Features:

  • Ligands - C8 and C18 options available
  • Flow rates - Both nano and micro flow rate columns are available

 

Research Applications:

Our Trapping Columns can be used for micro-flow applications:

  • LC-MS analysis of proteins
  • LC-MS/MS analysis of peptides and tryptic digests of proteins
  • Off-line solid phase extraction

Our Capillary Columns can be used for nano-flow applications:

  • LC-MS/MS analysis of tryptic digests of proteins
  • Peptide mapping experiments
  • LC-MS analysis of proteins

Product Specifications:
    
Capillary columns are packed with Monitor C8 and C18 beads for reproducible, high quality chromatographic performance in LC-MS applications.  Fabricated from standard 360um OD fused silica capillary and PEEK fittings, our Capillary Columns are specially designed to operate with the Protea Explorer System to improve LC-MS analyses.
    
Trapping Columns are packed with Monitor C8 and C18 beads for reproducible quality chromatographic performance in LC-MS applications. The compact cartridge design permits easy connection to either a switching valve, fitting assembly, or  union that utilizes a standard 10-32 format. This connection versatility permits the Trapping Columns to be used in traditional LC-MS applications, as well as for off-line experiments with the Protea Explorer System.

Base peak chromatogram from the LC-MS separation of 2-pmol angiotensin and somatostatin using a C8 Trapping Column and a 20-minute gradient with a flow rate of ~500-nL/min.

 

 

 
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