microfluidics separation products

MICROFLUIDIC CHIPS

Protea has established a strong design and production capability for microfluidic applications in protein, biology and chemistry research areas. We use fused silica substrate for our microfluidic chips to permit quality in situ observations of your experiments. Our microfluidic products are produced using both state-of-the-art photolithographic methods and other proprietary techniques developed in our labs. Our devices enable many diverse research applications, including capillary electrophoresis, cell culturing and autodilution. Each device has multiple uses, providing maximum opportunity to obtain useful data. We proudly offer Upchurch fittings as optional accessories for all devices.

DOUBLE T

Our Double T chip (TT) comes with two identical TT channels. To enhance the ease of your experiments, each structure is accompanied with a ruler and a dye concentration calibration channel. The channels are of a standard depth of 20µm (±15%) and width of 100µm (±15%) and 200µm TT length There are two options for connections: a standard reservoir of 80uL volume or a connection for 360µm fused silica tubing.
MIXER AND DILUTOR
Our Mixer and Dilutor devices are designed for fluid researchers interested in conducting mixing experiments involving different fluids or in performing crystallization studies. The chip dimensions are standard, but yet large enough to handle high flow rates (up to 1uL/min). Our proprietary flow restrictor can be used to capture grown crystals while allowing different media to pass through.
CELL RETAINER AND CELL CULTURE Our Cell Retainer and Cell Culture devices are designed for cell researchers interested in conducting cellular experiments by changing media or drug candidates and growth conditions. Dimensions are standard but yet big enough to accommodate many cells. Our proprietary cell strainer gently restricts cells from moving in fl owing media but still Our proprietary cell strainer gently restricts cells from moving in fl owing media but still allows them to be removed for other testing.
CUSTOM CHIP DESIGN Protea has pioneered a new way to facilitate providing custom Microfluidic Chips. Utilizing our UMOSTM (Universal Microfluidic Ordering System), any researcher can submit their design for production. Best of all, there is only a two (2) chip minimum order and turnaround time is typically 7-10 business days upon receipt of order. We can supply your laboratory with additional information to help you make an outstanding publication, including SEM images, profilometer scans, digital microscope images, as well as rulers and names for image identification. Simply add any of these on as an additional request.
For a complete presentation of our Microfluidic capabilities, go to www.microfluidics4biology.com. To Order your Microfluidic Chips, please use the following specification grid. (chips are available in all combinations of width and depth; fittings included.)

Catalog part number format: CH-ABC
Where A= Type of Chip, B= Width of Design, C= Depth of Design (please refer to grid below)

For Example: In order to order a TT Chip of width 100 µm and depth 20µm the part number will be: CH-123

(CH-123, where CH = chip, 1 = TT, 2 = 100 µm width, 3 = depth 20µm)

A= Type of Chip	Chip Type No. — Please see order grid below for full information
B= Width of Design	2= 100µm, 4= 200µm
C= Depth of Design	1= 15µm, 3= 20 µm

Chip Type Number	Name of Chip	Description	Pricing per Pair
1	TT	Standard double T structure with two sets of double T channels	$185/pack of 2
2	Auto Dilution	Standard chip with microfluidic channels to dilute solutions	$185/pack of 2
3	Mixing	Standard Microfluidic chip to perform various fluid mixing experiments	$185/pack of 2
4	Cell Growing	Chip to culture cells with observation under microscope	$185/pack of 2
5	Cell Retainer	Chip to isolate cells from a solution	$185/pack of 2
6	IVF	Standard chip to enhance the procedure of IVF	$185/pack of 2
7	CE-MS	Standard chip for Capillary Electrophoresis	$185/pack of 2
8	Nanoliter spotting slides	Micro-Array for spotting DNA	$420/pack of 10

Bio-fluidic Kit: Our Bio-fluidic kit provides a complete set of fittings, connections, and accessories to be used with our microfluidic, biology, and cell-based chip products. Each kit contains the following: 6-32 coned port and standard 6-32 nuts for 360um OD capillary, 1/4-28 port reservoir, port/chip seal-coned ports, port adhesive, 6-32 standard plug, 1-mL Luer-lock disposable syringe, 10-32 female to female Luer connector, adhesive, 6-32 standard plug, 1-mL Luer-lock disposable syringe, 10-32 female to female Luer connector, PTFE 0.012” ID capillary connectors, 2-um 25-mm syringe filter, 3-mL disposable transfer pipette, pipette hand PTFE 0.012” ID capillary connectors, 2-um 25-mm syringe filter, 3-mL disposable transfer pipette, pipette hand pump, disposable pipette, hose/pump connector, silicon hose, 1”x1” capillary cutter, 30cm of 75um ID/360um pump, disposable pipette, hose/pump connector, silicon hose, 1”x1” capillary cutter, 30cm of 75um ID/360um OD fused silica capillary, and a double lens magnifier. Additional information on our kits and individual components can be found on our website at: www.microfluidics4biology.com

Catalog Number	Description	Unit	Price per Unit
MK-002-BF	Bio-fluidic kit	1	$276