Component Products: Electroelute | Fractionate | Collect | Digest | Separate | Analyze
PILFerTM
Fractionate
Our in-line protein filter system (PILFer) is a highly flexible protein filter that provides a one-step means of placing an untouched sample directly into a separation or purification process, delivering rapid and reliable results. Protea’s PILFer system uses in-line molecular weight cut-off (MWCO) filter membranes for fractionation and has two selectable ports. Researchers can achieve true in-line protein purification, desalting and/or concentration directly from any solution. The PILFer system saves time and prevents sample loss from concentration in off-line devices. When coupled with the Protea Explorer System switching valves, researchers can achieve rapid injection of purified, filtered samples directly into an LC column or MS
Features:
- In-line format: Minimizes transfer losses and contamination from sample handling
- MWCO filters: Allows selective MW fractionation
- SDSaway: Permits removal of SDS detergent
Research Applications
The PILFer in-line protein filter system is recommended for applications where it is advantageous to directly inject samples into an HPLC or LC/MS system, including:
- Concentrating, purifying and desalting protein and DNA samples
- Purifying recombinant proteins
- Separation of proteins by molecular weight
- Removal of SDS detergent from protein samples using SDSaway reagent
Specifications:
| Effective filter area | 0.18cm2 |
| Filter storage temperature range | 50 – 80°F (19 – 29°C) |
| Volume capacity | 45 – 2000mL |
| Concentration capacity | 100 – 100,000pmol |
| Filtration flow rate (dependent upon MWCO filter used) | 15 – 120mL/min |
| Filtration time | = (sample volume/flow rate) X 2 |
| Retentate collection flow rate | 150 – 400mL/min |
| Retentate volume | 100mL minimum |
| Swept volume | 43mL |
| pH range | 1 – 14 |
| Recovery yield | >85% |
Mass spectrum of a PILFer fractionated mixture (upper spectrum) of the protein myoglobin and the peptides angiotensin I and somatostatin. The lower two spectra illustrate the effi cient fractionation of the mixture to remove the peptides (the fi ltrate) from the purifi ed protein myoglobin (the retentate) using a 10-kDa MWCO membrane.