Component Products: Electroelute | Fractionate | Collect | Digest | Separate | Analyze
DIGESTorTM Digest
Protea’s DIGESTorTM cartridges enable protein researchers to easily digest samples in a several minutes rather than a few hours. The DIGESTor cartridge contains a high density of immobilized trypsin enzymes on 20mm beads. Connecting the DIGESTor cartridge in-line reduces the potential for contamination, In addition, using the DIGESTor combined with Protea’s PILFer permits reduction and alkylation prior to digestion. Coupling this system to an LC or LC-MS makes the once tedious task of protein sequencing from tryptic in-gel digests simpler and less time-consuming. A standard 10-32 connection makes the DIGESTor connections compatible with all LC systems.
Features:
- Throughput: In-line format allows for rapid digestion
- Efficiency: High density enzyme bed improves efficiency of digestion
Research Applications
- Protein sequencing and identification
- Post translational modification characterization
- Protein – protein interaction studies
Product Specification
| Volume | 2 ml |
| Digestion Time | 5 – 10 min |
| Flow Rate | 0.1 to 1.0 mL/min |
| Pressure Range | 1 – 1000 PSI |
| Enzyme | Trypsin (bovine pancreas) |
| Temp. Range | 25 – 37o C |
| pH Range | 6.9 – 8.3 |
| Storage | 50 mM Tris/10 mM CaCl2 pH 8.0 |
| Load Concentration | 10 – 5000 pmol |
Base peak chromatogram from the LC-MS/MS analysis of a 20 pmol carbonic anhydrase protein digest mixture generated from processing of the sample with the DIGESTor cartridge. The inset mass spectrum shows a representative CID spectrum from the analysis. The table illustrates the high sequence coverage of the protein obtained from the digestion plus LC-MS/MS analysis.