DIGESTorTM               Digest

Protea’s DIGESTorTM cartridges enable protein researchers to easily digest samples in a several minutes rather than a few hours. The DIGESTor cartridge contains a high density of immobilized trypsin enzymes on 20mm beads.  Connecting the DIGESTor cartridge in-line reduces the potential for contamination, In addition, using the DIGESTor combined with Protea’s PILFer permits reduction and alkylation prior to digestion. Coupling this system to an LC or LC-MS makes the once tedious task of protein sequencing from tryptic in-gel digests simpler and less time-consuming. A standard 10-32 connection makes the DIGESTor connections compatible with all LC systems.

Features:

• Throughput:  In-line format allows for rapid digestion
• Efficiency:  High density enzyme bed improves efficiency of digestion

Research Applications:

• Protein sequencing and identification
• Post translational modification characterization
• Protein – protein interaction studies

Product Specifications:

Volume 2 ml Digestion Time 5 – 10 min Flow Rate 0.1 to 1.0 mL/min     Pressure Range  1 – 1000 PSI Enzyme       Trypsin (bovine pancreas) Temp. Range 25 – 37o C pH Range 6.9 – 8.3 Storage 50 mM Tris/10 mM CaCl2 pH 8.0 Load Concentration  10 – 5000 pmol     Base peak chromatogram from the LC-MS/MS analysis of a 20 pmol carbonic anhydrase protein digest mixture generated from processing of the sample with the DIGESTor cartridge. The inset mass spectrum shows a representative CID spectrum from the analysis. The table illustrates the high sequence coverage of the protein obtained from the digestion plus LC-MS/MS analysis.