We create new integrated proteomics technology, by developing new solution-based (gel-free) methods, coupled with ultrasensitive mass spectrometry. We offer integrated Protein identification and post-identification services, including Protein characterization, identification of posttranslational modifications, and protein-protein interactions. Our high quality results are achieved by directly coupling nanoflow HPLC with microelectro spray ionization on a Thermo Finnigan LCQ DECA XP ion trap mass spectrometer.
Our integrated Protein Identification services are available both for your samples (gel bands or solution), or you may request samples for your use from our own protein sample database.
Our Integrated Proteomic Services:
We are known for our ultrasensitive and validated Mass Spectrometry protocols integrated with innovative sample optimization and protein separation methods.
For Protein Identification
Protein identification is validated by manual confirmation of the MS/MS spectra. Further comparison is available, of peptide fragmentation pattern and/or co-elution experiment with synthetic standard
Identification of proteins in complex mixtures using immunoprecipitation, SDS-PAGE and/or 2D/LC.
Identification of proteins from SDS – PAGE and 2D Gels with enhanced data validation
We will look at phosphopeptide mapping of your protein, using IMAC and LC/MS/MS. We will identify candidate binding partners for known targets.
For Protein Characterization
– a human cancer protein screening service, of over 200 highly-characterized total protein samples (dot blot). (Click here to order)