Case Studies and App Notes

New Case Study
CASE STUDY: Direct Characterization of Cysteine Binding in Signal Transduction Pathways by LC MS-MS

The characterization of disulfide-bridges and cysteine binding in proteins provided incredible insight to investigators focused on developing innovative biotherapeutic strategies (such as monoclonal antibodies). Using mass spectrometry to determine a protein therapeutic’s higher order structure and confirm its proper folding is quickly becoming an industry standard for describing the critical relationship between structure, function, and efficacy. Protea’s Bioanalytical Services team employs the most advanced mass spectrometry-based techniques and instrumentation to offer protein characterization studies which directly describe a protein’s three-dimensional structure as well as its structure-function relationships. In addition, this specificity of cysteine binding has been shown to play an important role in the regulation of protein-protein interactions and signal transduction pathways in biological systems.

This case study highlights the utility of high-resolution mass spectrometry analysis for the direct characterization of cysteine binding for protein-protein interactions. Data from a recent publication featuring work performed by Protea for one of our academic collaborators, conclusively demonstrated cysteine binding between Src SH2 domains and the cytoskeletal protein cortactin, presenting a new paradigm for SH2 domain functionality in domain-based signal transduction.

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Case Studies:
Label-Free Quantitation of Proteins in Human Prostate Cancer Patient Plasma CASE STUDY: Biomarker Discovery in Human Prostate Cancer Patient Samples Using Combined MuDPIT and BIOiTRAQ Workflows
CASE STUDY: Biomarker Quantitation in Human Plasma by Multiple Reaction Monitoring (MRM) CASE STUDY: Disulfide Bridge Mapping by LC MS-MS
CASE STUDY: Phosphorylation Site Characterization CASE STUDY: Human Plasma Biomarker Discovery by MuDPIT
CASE STUDY: Biomarker Discovery by BIOiTRAQ
Application Notes:
Quantitation of a Topically Dosed Pharmaceutical in Skin Tissue by MALDI-MS Imaging Tissue Microarrays for High Throughput Mass Spectrometry Imaging and Classification of Human Disease Tissue – A Clear Cell Renal Cell Carcinoma Example
Multi-modal Mass Spectrometry Approach to Characterize Clozapine and Metabolites by Combining Imaging Modalities and Absolute Quantitation Histology Guided Mass Spectrometry: A New Analytical Workflow for Clinical Research and Biomarker Discovery
Drug Distribution Studies by Mass Spectrometry Imaging (MSI) using the LAESI DP-1000 System Fast and Comprehensive Characterization of Human IgG1 by High-Resolution Accurate-Mass Mass Spectrometry
Molecular Imaging of Animal and Human Tissue Samples by LAESI-MS In vivo mass spectrometry with the LAESI DP-1000 system: A new tool for native biomolecular profiling and biomarker discovery
Imaging Lipids in Mouse Brain Tissue Using the Protea LAESI DP-1000 System LAESI-MS vs MALDI-MS Imaging: Lipid Imaging from Mouse Brains
Stability Analysis of the Human IgG1 Antibody Cross Platform Lipid Imaging of Silver Nanoparticle-Exposed Rat Brain by LAESI-MS and MALDI-MS
Quantitative Mass Spectrometry Imaging of Rabbit Skin Topically Dosed with an Antifungal Compound using a Stable Isotope Labeled Internal Standard In situ Detection and Imaging of Ergot Alkaloids in Ipomea tricolor by LAESI-MS-MS and MALDI-MS
Integration of MS Imaging and Proteomic Data for Biological Discovery in a 3D Microtissue Model of Colon Cancer In situ Top Down Proteomics: Targeted Imaging of Proteins in Tissue Using Laser Ablation Electrospray Ionization Mass Spectrometry (LAESI-MS)
A Novel Web Interface to Facilitate Histology Guided Mass Spectrometry Proteomic Analysis in the Lung and Brain of Rats Following Silver Nanoparticle Inhalation
Histology Guided Mass Spectrometry Determines Proteomic Differences Associated with Carbon Nanotube Exposure in the Lung Combining Laser Ablation Electrospray Ionization Mass Spectrometry with Collision Cross Section for Improved Metabolite and Lipid Identification from Clinical Samples
Mass Spec Imaging Differentiates Histological Subtypes of Stage 1 Pulmonary Adenocarcinoma Direct Analysis of Animal and Plant Tissues by LAESI-MSI with Ion Mobility for Mapping of Metabolites and Lipids
Direct Sample Analysis Without Extraction Using LAESI-MS 2D and 3D Direct Analysis of Plant Tissues
Mass Spectrometry Imaging of Raclopride and Fexofenadine in Whole Body Mouse Tissue Using LAESI-MS A Lipidomic Imaging Analysis of Mouse Brain Using LAESI-MS in Positive and Negative Ion Modes Coupled with Ion Mobility Spectrometry
LAESI Mass Spectrometry Imaging of Contact Lens Spoilage Online Core-Shell HPLC Protein Separation and Top-Down Proteomics Using High Resolution Mass Spectrometry
Protein Profiling of Animal Tissues Using LAESI-MS
…concepts into knowledge