εtipTM ESI Tip     Analyze

Protea has pioneered improvement of the ESI process by engineering a revolutionary spray tip that directs ions to the Taylor cone surface, creating smaller droplets with more uniform charge distribution and less hydratioΕn. The enhanced ESI process leads to a reduction of background noise in your spectra, with an average improvement of 50%, compared to a pulled fused silica tip. This improved process is fully functional at both micro-ESI (0.4 to 5.0mL/min) and nano-ESI (50 to 400nL/min) flow rates. The etip construction is nearly unbreakable and has a longer lifetime than traditional pulled fused silica tips.

Two standard proteins, cytochrome C and myoglobin, were analyzed by direct infusion ESI-MS in a Finnigan LTQ-MS using the Protea εtip and a New Objectives fused silica PicoTip (30um tip ID) at a concentration of 10-pmol/μL and a fl ow rate of 1.0-μL/min. The Total Ion Current (TIC) plots of these analyses show that the εtip demonstrates a more stable ESI performance (baseline RSD) and a general increase in sensitivity that are realized from the εtip’s more efficient electrospray ionization.