Component Products: Electroelute | Fractionate | Collect | Digest | Separate | Analyze
ΕtipTM ESI Tip Analyze
Protea has pioneered improvement of the ESI process by engineering a revolutionary spray tip that directs ions to the Taylor cone surface, creating smaller droplets with more uniform charge distribution and less hydratioΕn. The enhanced ESI process leads to a reduction of background noise in your spectra, with an average improvement of 50%, compared to a pulled fused silica tip. This improved process is fully functional at both micro-ESI (0.4 to 5.0mL/min) and nano-ESI (50 to 400nL/min) flow rates. The etip construction is nearly unbreakable and has a longer lifetime than traditional pulled fused silica tips.
Features:
- ESI Performance – Enhanced ionization improves signal-to-noise ratio
- Spray Position – Adjustable tip holder allows variable angle spray
- Durability – New space age composite makes tips resistant to breakage and clogging
Research Applications
- Direct infusion ESI-MS investigations
- LC-MS/MS analyses of peptide and protein digest mixtures
- Elecrospray ionization efficiency studies
Product Specification
| Flow rate | 0.050 – 5 ml/min |
| Voltage range | 1.5 – 6.0 kV |
| Working Distance | Range 2 – 8mm, optimum 5 mm |
| Solvent compatibility | No known incompatibilities |
| Polarity | Positive and negative |
B- Taylor cone
C. Plume
Two standard proteins, cytochrome C and myoglobin, were analyzed by direct infusion ESI-MS in a Finnigan LTQ-MS using the Protea εtip and a New Objectives fused silica PicoTip (30um tip ID) at a concentration of 10-pmol/μL and a fl ow rate of 1.0-μL/min. The Total Ion Current (TIC) plots of these analyses show that the εtip demonstrates a more stable ESI performance (baseline RSD) and a general increase in sensitivity that are realized from the εtip’s more efficient electrospray ionization.