Protea Research Laboratory

About

Protea’s Protein Research Laboratory is a state-of-the-art mass spectrometry facility, featuring MALDI TOF/TOF and linear trapping quadrupole (LTQ) mass spectrometers. Combined with our experience in advanced protein sample preparation methodologies, Protea is positioned to offer a variety of protein identification and characterization services for both gel-based and solution-based samples. Our mission is to provide you with the very best MS data from your valuable protein samples.

INTRODUCING gelSTAT Services
Get high quality mass spectrometry data on your gel samples – STAT

gelSTAT services let you obtain peptide mass fingerprinting and LC-MALDI mass spectrometry data within seven days*, including a complete data report. MS data is generated using our ABI 4800 MALDI TOF/TOF mass spectrometry.

BENEFITS OF USING gelSTAT SERVICES:

  • Complete data reports are provided with all gelSTAT services
  • Sample Transport Kits, including destaining reagents, are provided without charge
  • Shipping charges for sending your samples to Protea are prepaid by us (via FedEx)
  • You will receive electronic notifications when your samples are received, and when your data reports are available for download by your laboratory.
  • Mass Spectrometric data is generated using ABI’s 4800 MALDI TOF/TOF and nano-flow LC-MALDI spotter.
*Results (summary report and data files) will normally be provided within five working days.

We utilize an integrated approach to services, focusing on both upstream sample preparation and downstream mass spectrometric analysis. All of our service work is conducted on site in our Protein Research Laboratory. Our secure facility features HEPA-filtered air and a climate-controlled environment.

Our protein mass spectrometrists are highly experienced with the newest technologies, including ultra-high performance liquid chromatography (UHPLC), nano-flow LC MALDI spotting, gel electroelution of proteins, and BIOiTRAQ comparative analyses. For high throughput protein identification, MascotTM search engine is used for peptide mass fingerprinting in which protein samples of interest are enzymatically digested, desalted and analzed by MALDI-TOF mass spectrometry. The peptide mass fingerprint from the spectrum (example shown in Figure 1A is compared to “virtual” fingerprints obtained by theoretical cleavage of protein sequences stored in databases, and high scoring peptides are reported as possible candidates. Protein PilotTM and SequestTM search engines are used to analyze LC-MALDI MS/MS and LC-ESI-MS data respecively. Our clients receive the benefits of our strong core competencies in protein biochemistry and analytical chemistry and of our commitment to improving existing sample preparation technologies.

Protea is unique as a service provider, because of our leadership in the development of improved sample preparation methodologies for both gel- and solution-based samples. Solution-based proteomics streamlines sample preparation and analysis, resulting in minimized sample loss and contamination. We can employ new robust and reproducible sample preparation procedures.


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Features

Featured Services

  • Protein identification and characterization
  • BIOiTRAQ quantitative analysis of related samples
  • Peptide mass fingerprinting
  • 1D- and 2D-LC-MS/MS analysis
  • Post-translational modification analysis (e.g. phosphorylations)
  • Gel electroelution and protein extraction/purification
  • De novo sequencing
  • Phosphoprotein and phosphopeptide analysis
  • NEW - gelSTAT protein I.D. from gel samples

FEATURED SOFTWARE PLATFORMS Protein Identification

  • Mascot (Matrix Sciences)
  • Protein Pilot (Applied Biosystems)
  • BioWorks (Thermo Finnigan)
  • GPS Explorer (Applied Biosystems)

De novo Sequencing

  • PEAKS Studio (Bioinformatics Solutions)

Protea Sample Preparation Products used in services

  • Recovery of intact proteins from 2D gel plugs
  • Functionalized pipette tips and SpinTips
    • Reversed phase C18, C4, C8
    • ZIC©-HILIC
    • Titanium Dioxide
    • Strong Cation Exchange (SCX)
  • Recrystallized MALDI Matrices
  • Immobilized enzme digestion beads
  • Protein and Peptide MS Standards
  • FACET microfilters and trapping columns
  • ProteaGels - ready-to-use gel solution

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Data Sets

A. Peptide mass fingerprint from the digest of 5 pmol ovalbumin analyzed by reflectron MALDI-TOF MS, with a sequence coverage of 48%.

B. Protein mass spectrum of 1 pmol myoglobin analyzed by linear mode MALDI-TOF MS

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Availability

gelSTAT
Protein and Peptide Identification Services - STAT**
Option 1: PMF
Peptide Mass Fingerprinting		 $400/set of 5 samples
Option 2: LC-MALDI + PMF
LC-MALDI and Peptide Mass Fingerprinting		 $750/set of 5 samples
MALDI mass spectrometry
Applied Biosystems, Inc. 4800 BIOiTRAQ MALDI TOF/TOF mass spectrometer
LC-MALDI MS/MS analysis, 30 minute gradient *
protein identification and characterization		 $300/sample
2D LC-MALDI MS/MS analysis, 8 step MudPIT *
(strong cation exchange + C18 reversed phase)
protein identification and characterization		 $1000/sample
In-gel digestion + LC-MALDI MS/MS analysis of a gel-based sample *
protein identification and characterization		 $250/sample
Phosphopeptide enrichment + LC-MALDI MS/MS analysis of a protein digest *
phosphopeptide mapping, protein identification and characterization		 $250/sample
BIOiTRAQ LC-MALDI MS/MS analysis
relative quantitative analysis + protein identification and characterization		 $750-1000
per sample
BIOiTRAQ 2D LC-MALDI MS/MS analysis, 8 step MudPIT
relative quantitative analysis + protein identification and characterization		 $1200-2000
per sample
Custom MALDI or LC-MS/MS analysis
protein identification and characterization, small molecule analysis		 by contract
Electrospray Ionization (ESI) mass spectrometry
Thermo Electron LTQ-XL Linear ion trap mass spectrometer
Direct infusion analysis
molecular weight determination, complexity analysis		 $150
LC-MS/MS analysis, 30 min. gradient *
protein identification and characterization		 $300
LC-MS/MS analysis, 30-90 min. gradient *
protein identification and characterization		 $500
2D LC-MALDI MS/MS analysis, 8 step MudPIT *
(strong cation exchange + C18 reversed phase)
protein identification and characterization		 $1500
In-gel digestion + LC-MS/MS analysis of a gel-based sample *
protein identification and characterization		 $400
Custom LC-MS/MS analysis
protein identification and characterization, small molecule analysis		 By Contract
Other Services
Available Through the Protein Research Laboratory Gel Sample Analysis
Gel electroelution + protein extraction/purification *
for protein molecular weight determination and top-down proteomics applications		 $50
Gel electroelution, protein extraction/purification, + MALDI analysis
for protein molecular weight determination and degradation product analysis		 $200
Consultation
for project design and protein research plan development		 $150/hr

* - Sample must be "ready-for-digestion"; additional sample prep prior to digestion will be preformed by contract only and at addition cost.
** - Results (summary report and data files) will normally be provide within five working days.

Prices are subject to change without notice.

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