Instructions for sending us your samples.
- a western blot human cancer profiling service , for use with our pre-designed , tumor-specific western blots, or you design your own western blots, from our sample database
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Our human cancer-specific Western blot panels are used to determine cell signaling and protein expression patterns in specific disease and conditions. We offer screening on over 50 predesigned protein panels, each specific to multiple stages of breast, lung, colorectal, and prostate cancer, and other disease. Also, use our Designer western service, and make your own sample selections from our total protein database. Clinical and Pathology data available for each sample.
Our total protein samples are derived from fresh-frozen, Pathologist-reviewed , individual tissue samples, Dnase-treated, and assessed for protein integrity on the Agilent/Caliper system (data provided), samples are applied to SDS_PAGE gels, and transferred to either PVDF or nitrocellulose membranes(your choice). Use our pre-designed western blots, or design your own, to determine the size, abundance and phosphorylation patterns of specific proteins in highly-characterized samples.
We will send you the western blots for use in your lab, or screen your antibody on the blots you select or design. Each western blot contains 14 individual samples plus molecular markers.
Depending on the specificity of the protein targets, we will use your primary antibody to determine the size, abundance and phosphorylation status of the target proteins.
The complete digital image will be provided, along with an interpretation of the results. A film copy is available on request, without charge.
Agilent/Caliper data will be provided on all samples, to document protein integrity.
Clinical and Pathology data for the individual protein samples will be provided
Densitometric reading and plotted results are available on request
Service - We screen your antibody as follows:
-1 selected western blot - $1,200
-2 selected western blots - $2,100
-3 selected western blots - $2,900
>3 selected western blots - please inquire